Code generated in the video can be downloaded from here:
https://github.com/bnsreenu/python_fo...

Step by step...
Refer to the next tutorial for a single step function that does the job.

Steps...
#Step 1: gaussian blur the image and detect maxima for each nuclei
#Step 2: threshold the input image after applying light gaussian blur (sigma=1)
#Step 3: Exclude maxima locations from the background, to make sure we only include the ones from nuclei
#Step 4: Separate maxima locations into labels using masked voronoi
#Step 5: Separate objects using watershed.

For installation instructions of the pyclesperanto package,
please refer to the following link
https://github.com/clEsperanto/pycles...