In our previous videos we introduced the CRISPR Cas9 system briefly (   • CRISPR Cas9  A Brief Introduction  ) and presented the different tools and methods available for their expression in living systems (   • 2) CRISPR Cas9  Methods and Tools  ). Here we will go over the design criteria of the gRNA and introduce you to the online programs that simplify this procedure. We invite you to watch our previous videos first before continuing with this one.

For more information on how to design gRNA for CRISPR Cas9 systems, please visit:
➜ Knowledge Base: https://info.abmgood.com/crisprcas9...

Chop Chop Harvard: https://chopchop.rc.fas.harvard.edu/

*Please note: There is an error at ~1:12. Cas9 has an HNH and an RuvC1like nuclease domain (in the video, it was mislabeled as "NHN").

Want more tips, tricks, guides, and protocols on how to knockout a gene using CRISPR? Join our free, 4week #CRISPRCrashCourse: http://info.abmgood.com/crisprcrash...

For more resources on CRISPR, visit:
➜ https://info.abmgood.com/crisprcas9

*Spanish subtitles are also available

Watch the other videos in this series on CRISPR:
➜ Introduction:    • CRISPR Cas9  A Brief Introduction  
➜ Methods and Tools:    • 2) CRISPR Cas9  Methods and Tools  
➜ CRISPR Playlist:    • CRISPR Cas9  A Brief Introduction  

Check out our other video series:
➜ Adeno Associated Virus:    • 1) Adeno Associated Virus (AAV)  An ...  
➜ Next Generation Sequencing:    • 1) Next Generation Sequencing (NGS) ...  

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