Benedict's Test This is the video on benedict's test which done for the detection of reducing sugars present in the urine.
Video contains Principle of Benedict's Test, Procedure, Discussion of viva questions and Demonstration of benedict's test.

Reducing Sugars 00:28 minutes
Benedict's Test Principle 00:52 minutes
Benedict's Test for Glucose, Sucrose and Fructose 01:49 minutes
Color changes seen in benedict's test 02:13 minutes
Composition of benedict's reagent 02:38 minutes
Benedict's test procedure 03:06 minutes
Benedict's test is a semi quantitative test 03:27 minutes
False positive benedict's test 04:55 minutes
Demonstartion of benedict's test 05:33 minutes

All reducing sugars give positive benedict’s test.

Principle Reducing sugars under hot alkaline conditions tautomerize and form enediols which are powerful reducing agents.

They reduce the cupric ions of Benedict’s reagent to red cuprous oxide.
Enediol is the enol form of sugar because two OH groups are attached to the double bonded carbon

Color Changes from Blue to Green to Yellow to Orange to brick red if there is presence of reducing Sugar. Sucrose will give negative benedict’s test

Benedict’s qualitative reagent contains:
1. Copper sulfate: Furnishes cupric ions (Cu++) in solution.
2. Sodium carbonate: Provides alkaline medium.
3. Sodium citrate: It acts as a preservative.

Procedure –
5 ml of Benedict’s reagent + 58 drops of solution
Apply heat to it for 12 minutes and observe
the color changes

#glucosuria #sugarinurine #benedict's #benedicttest #biochemistrybasicsbydramit #biochemistrypractical #diabetes