This video explains about Bradford Assay | Bradford Test Protein Estimation Principle, Protein calculation.

Simple method for the Determination of unknown protein concentration from standard curve in excel
   • How to calculate Protein Concentratio...  

The Bradford protein assay is a colorimetric assay for the determination of protein concentration, It is dependent on the amino acid composition of the measured protein. The Bradford protein assay was developed by Marion M. Bradford. The Bradford protein assay is a very popular protein assay method because it is simple, rapid, inexpensive and sensitive.

The principle is based on the shift in absorbance maximum of acidic solution of coomassie brilliant blue dye from 465nm to 595nm when binding to the protein occurs.

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