Inspired by Thatcheritescot's original video covering the same topic. I decided to update it since the clips were in very poor quality, they were missing Margaret Beckett's PMQs to John Major, and was posted in 2012 so there were many new leaders to add.

Timestamps (denoted as PM/Opposition):
Thatcher/Kinnock (first televised) 0:00
Thatcher/Kinnock (last) 2:20
Major/Kinnock (first) 4:30
Major/Kinnock (last) 7:13
Major/Smith (first) 12:18
Major/Smith (last) 16:10
Major/Beckett (first) 19:15
Major/Beckett (last) 21:58
Major/Blair (first) 24:43
Major/Blair (last) 27:56
Blair/Major (first) 35:49
Blair/Major (last) 40:32
Blair/Hague (first) 48:20
Blair/Hague (last) 53:15
Blair/Duncan Smith (first) 1:00:43
Blair/Duncan Smith (last) 1:07:48
Blair/Howard (first) 1:14:11
Blair/Howard (last) 1:22:01
Blair/Cameron (first) 1:25:43
Blair/Cameron (last) 1:31:02
Brown/Cameron (first) 1:37:15
Brown/Cameron (last) 1:46:35
Cameron/Harman (first) 1:58:13
Cameron/Harman (last) 2:06:52
Cameron/Miliband (first) 2:14:46
Cameron/Miliband (last) 2:27:27
Cameron/Harman 2 (first) 2:34:05
Cameron/Harman 2 (last) 2:40:51
Cameron/Corbyn (first) 2:54:29
Cameron/Corbyn (last) 3:08:08
May/Corbyn (first) 3:20:49
May/Corbyn (last) 3:32:16
Johnson/Corbyn (first) 3:44:42
Johnson/Corbyn (last) 3:59:46
Johnson/Starmer (first; Starmer had already appeared as Opposition Leader against Dominic Raab as Johnson was diagnosed with COVID) 4:11:10
Johnson/Starmer (last) 4:22:49
Truss/Starmer (first) 4:34:49
Truss/Starmer (last) 4:43:29
Sunak/Starmer (first) 4:50:34