The CRISPR/Cas9 system has recently emerged as the most powerful gene editing method to study gene function. This new technology has made gene editing easy to do in any lab. To perform CRISPR/Cas9mediated gene editing, the first step is to design guide RNA sequences for the target gene. In this webinar, we will present detailed gRNA design principles and provide stepbystep guidance on how to design high specificity gRNAs that avoid offtarget effects. We will also introduce the online gRNA design tool and other resources offered by GenScript to help you start doing CRISPRmediated gene editing with the knowledge and tools you need to be successful.