Disney Junior – Live on Stage! (originally Playhouse Disney – Live on Stage! until 2011) is a live show attraction featuring puppet characters from Disney Junior's television programs, located at three Disney theme parks: Disney's Hollywood Studios at the Walt Disney World Resort in Florida, Disney California Adventure at the Disneyland Resort,[2] and Walt Disney Studios Park at Disneyland Paris.

The show, as of 2017, currently consists of characters from Mickey Mouse Clubhouse, Sofia the First, Doc McStuffins, and Jake and the Never Land Pirates. Prior to Disney Junior – Live on Stage!, the theater was the Soundstage Restaurant from the park's opening date until November 14, 1998; the restaurant featured sets over the years from Big Business, Beauty and the Beast, and Aladdin. Following that, the theater hosted Bear in the Big Blue House – Live on Stage from June 7, 1999 until August 4, 2001.

The current edition of Disney Junior Live! (which was the third and final edition of Playhouse Disney Live! as it was before) begins with a cast member named Casey (he or she) running on stage while the Disney Junior theme song plays. Casey then asks the audience to yell out their names. While everyone is yelling, Mickey Mouse himself yells out his name, and then invites the audience to his clubhouse. As guests arrive at the clubhouse, Mickey and Casey sing the Mickey Mouse Clubhouse theme song, during which Donald, Daisy, Goofy and Minnie also appear.

At the clubhouse, Mickey informs guests that it's Minnie's birthday. In order to help out, Daisy agrees to hang up a "happy birthday” sign, Goofy agrees to make a cake, and Donald says that he can write a birthday song. The three then explain that it is going to be difficult for them to accomplish their tasks. Mickey and Casey agree that the best way to solve your problems is to study stories. In order to hear some stories, Mickey and his friends call on Tootles to give them tools to help them. The three items Tootles carries, relate to other Disney Junior shows: Sofia the First, Doc McStuffins, and Jake and the Never Land Pirates.