Estimation of Serum Total Cholesterol by Enzymatic (CHODPOD) Method This is the video on estimation of serum total cholesterol by cholesterol oxidase and peroxidase method with discussion of viva questions and
demonstration.
Aim and Introduction 00:30 minutes
Importance of estimation of cholesterol 01:05 minutes
Important biological compounds derived from cholesterol 01:48 minutes
Principle of Cholesterol Estimation by Enzymatic Method 02:22 minutes
Operational Procedure 03:11 minutes
Calculation of Total Cholesterol 03:55 minutes
Normal Range of Total Cholesterol 04:38 minutes
Lipid Profile 04:43 minutes
Friedewald equation 05:15 minutes
Hypercholesterolemia 07:25 minutes
Hypocholesterolemia 07:51 minutes
Viva Questions 08:11 minutes
Demonstration of Estimation of Serum Total Cholesterol by Enzymatic (CHODPOD) Method 11:17 minutes

Cholesterol is the most abundant steroid in the human tissue and body fluids.
It is synthesized in the liver and transported in the blood mainly in the form of LDL and HDL.
In blood, cholesterol is present in two forms:
Free (20 25%) and
Esterified (75 – 80%).

Important compounds derived from cholesterol:
Bile acid
1ry bile acid Cholic acid & Chenodeoxy cholic acid
2ndry acid Lithocholic acid & Deoxycholic acid
2. Vit.D
3. Steroid hormones


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