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Glycolysis is the process of breaking down glucose. Glycolysis can take place with or without oxygen. Glycolysis produces two molecules of pyruvate, two molecules of ATP, two molecules of NADH, and two molecules of water.

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Glycolysis can occur with or without oxygen. In the presence of oxygen, glycolysis is the first stage of cellular respiration. In the absence of oxygen, glycolysis allows cells to make small amounts of ATP through a process of fermentation.
Glycolysis takes place in the cytosol of the cell's cytoplasm. A net of two ATP molecules are produced through glycolysis (two are used during the process and four are produced.