This video tutorial is part of the Mastering qPCR Online Course in The Top Tip Bio Academy.

MASTERING QPCR ONLINE COURSE
https://courses.toptipbio.com/p/maste...

In this video tutorial, I will show you how to perform the deltadelta Ct method by using Microsoft Excel.

The deltadelta Ct method is a simple formula used in order to calculate the relative fold gene expression of samples when performing realtime polymerase chain reaction (also known as qPCR).

THE ONLINE GUIDE
http://toptipbio.com/deltadeltactpcr/'>http://toptipbio.com/deltadeltactpcr/

VIDEO BREAKDOWN
Step 1: Average the Ct values (00:53)
Step 2: Calculate delta Ct (01:59)
Step 3: Calculate the average delta Ct for controls (02:46)
Step 4: Calculate deltadelta Ct (03:39)
Step 5: Calculate 2^(deltadelta Ct) (04:54)

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