Video on Colorimeter and Beer's Lamber's Law This is video on introduction to colorimeter and HANDS on colorimeter which will be useful for 1st year MBBS as well as to DMLT students for the biochemistry practical.
Video contains
1. Introduction to Colorimeter 01:00 minutes
2. Beer's Lambert's Law 1:25 minutes
3. Absorbannce and OD 2:44 minutes
4. Parts of Colorimeter 4:00 minutes
5. Operational procedure 5:45 minutes
6. Different types of blank 6:52 minutes
7. Calculation 7:25minutes
8. HANDS ON COLORIMETER 8:39 minutes
Colorimetry
In this technique , the concentration of compound is determined by measuring the intensity of color.
Colorimeter
They are simple instrument which measure the intensity of transmitted light through color solution
Beer’s law
When a parallel beam of monochromatic light is passed through a solution, the absorbance (A) of the solution is directly proportional to concentration (C) of the substance in solution
A = KC
K is a proportionality constant
Lambert’s Law
When a parallel beam of monochromatic light is passed through a solution, the absorbance (A) of the solution is directly proportional to length of the light path (l)
A=Kl