This video is a must watch for beginners to understand how molecular cloning works. All steps of a molecular cloning assay are explained here!
References:
Helpful blog article: https://blog.addgene.org/plasmids101...
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Music:
Aakash Gandhi – Liquid Time (YouTube Audio Library)
Listen: • Aakash Gandhi Liquid Time
0:00 Intro
0:39 Vector generation
1:59 Insert generation
2:37 Isolation of vector and insert
3:48 Assembly
4:29 Transformation
4:51 Selection and screening
5:26 Verification
#molecularbiology #cloning #genecloning