Molisch Test for Carbohydrates This is the video on Molisch Test which is the general tests for carbohydrates. It is done to detect the presence of carbohydrates in the solution. Molisch test is explained in 5 minutes.
Video contains
Molisch Test Principle 00:28 minutes
Furfurals and 5hydroxymethylfurfurals 01:00 minutes
Molisch Test Reagents 01:24 minutes
Molisch Test Procedure 01:28 minutes
Will glyceraldehyde give molisch test ? 02:22 minutes
Demonstration of Molisch Test for Carbohydrates 02:45 minutes

It is a general test for all carbohydrates

Principal – Carbohydrates when treated with concentrated H2SO4 undergo dehydration to give furfural derivatives.
These compounds condense with alphanaphthol to form reddish violet colored products.

Pentoses yield furfurals and hexoses yield 5hydroxymethylfurfurals.

Reagent
1. Molisch’s reagent (alphanaphthol in 95% ethanol)
2. Concentrated sulfuric acid.

Procedure –
To 2 ml of carbohydrate solution, add 12 drops of 1% alcoholic alpha naphthol solution and mix. To this, add 2 ml of concentrated sulfuric acid along the side of the test tube to form a separate layer.

A violet ring is formed at the junction of two layers.

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