Learn how to get Free YouTube subscribers, views and likes
Get Free YouTube Subscribers, Views and Likes

Molisch's Test for Carbohydrates 2.0

Follow
Professor Drew Collop

Molisch's Test is used to identify a Carbohydrate.
5 drop of Molisch Reagent (alphanaphthol) was added to 2 mL of each sample.
2 mL of Sulfuric Acid was gently added to each test tube to layer the 2 solutions.
It is important not to mix the 2 solutions as the chemical reaction will occur at the interface.
The Sulfuric Acid converted the Carbohydrate into an aldehyde, which will then react with the alphanaphthol to create a purple or violet colour.

Purple colour change = Carbohydrate
Negative Control = Distilled Water
Positive Control = 1 % (w/v) Glucose
Unknowns Tested = 13

Alphabetical list of Samples all 1% (w/v) or (v/v):
Acetic Acid, Albumin, Arabinose, Fructose, Galactose, Glucose, Glycerol, Glycogen, Lactose, Maltose, Starch, Sucrose, Xylose

posted by Biagianti7d