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Analysis of amino acids is required in several areas of research, and it is a fundamental tool in product analysis. The application imposes different requirements on the analytical method because the amino acids play different roles.

Amino acids are the basic constituents of proteins. Qualitative and quantitative analysis of the amino acid composition of hydrolyzed samples of pure proteins or peptides is used to identify the material and to directly measure its concentration
Amino acids are also intermediates in metabolic pathways, often not directly involving proteins. The amino acids are measured as elements of physiological and nutritional studies. This has proven particularly important in monitoring the growth of cells in cultures, as used in the production of biopharmaceuticals . Similar considerations lead to the analysis of foods and feeds to ensure that nutritional
requirements are met.