Subcellular Fractionation is also known as cell fractionation, the process used to separate cellular components while preserving the individual functions of each component.
This is a method that was originally used to demonstrate the cellular location of various biochemical processes. Other uses of subcellular fractionation are to provide an enriched source of protein for further purification and facilitate the diagnosis of various disease states.
This lecture is divided into the following topics:
00:00 Introduction
00:35 Subcellular Fractionation
01:16 Extraction
01:59 Homogenization
02:21 Centrifugation
04:32 Centrifugal Fractions
04:52 Assessment of Purity
Some illustrations and information sourced from:
Koolman, Color Atlas of Biochemistry, 3rd edition: https://amzn.to/3iBo63T (eBook available)
Lieberman, Michael, Marks, Allan. Marks’ Basic Medical Biochemistry, A clinical Approach, 5th Edition: https://amzn.to/2Vyab5L (eBook available)
Murray, R. D.KVW Rodwell: et al Harper’s Biochemistry, 31st edition: https://amzn.to/3lLCBDY (eBook available)
Champe, P. Lippincott’s Illustrated Reviews: Biochemistry, 7th edition: https://amzn.to/3ywran3 (eBook available)
This is an episode of BiochemSerye entitled "Part 4 Subcellular Fractionation". This is part of a lecture entitled "Introduction to Biochemistry and Biochemical Aspects of the Cell". This is the introductory lecture given to firstyear Biochemistry students of the Faculty of Medicine and Surgery of the University of Santo Tomas.
Introduction to Biochemistry and the Biochemical Aspects of the Cell Playlist: • Introduction to Biochemistry and Bioc...
Full Video (Video Length 1 hours and 15 minutes): • Introduction to Biochemistry Lecture;...
Other Episodes of the BiochemSerye "Introduction to Biochemistry and Biochemistry of the Cell":
1. Part 1 Introduction to Biochemistry (Video Length 15 minutes and 28 seconds): • Introduction to Biochemistry and Biom...
2. Part 2 Chemical Components of Cells (Video Length 18 minutes and 50 seconds ): • Chemical Components of the Cell Bio...
3. Part 3 Physical and Chemical Properties of Water (Video Length 8 minutes and 20 seconds): • Part 3 Biochemistry of Water Bioc...
4. Part 4 Subcellular Fractionation (Video Length 6 minutes and 12 seconds): • Subcellular Fractionation / Cell Frac...
5. Part 5 Biochemistry of the Cell (Video Length 22 minutes and 43 seconds): • Part 5 Biochemistry of the Cell B...
6. Part 6 Compartmentalization (Video Length 2 minutes and 31 seconds ): • Part 6 Compartmentalization Bioch...
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