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The 5 steps of a solid phase extraction (SPE)

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SiliCycle Inc.

Solidphase extraction (SPE) is a technique designed for rapid sample preparation, purification and/or concentration prior to chromatographic analysis.

SPE uses a sorbent that has a strong affinity for the desired compound and solvent changes to collect the individual components as single fractions.
After applying the sample to a pre conditioned column, the column is eluted with a solvent that has a weak affinity for the desired product to removed impurities. It is then eluted with a solvent that has a strong affinity for the desired product thereby displacing it from the sorbent.

Common sample matrices include:

• human and animal biological fluids
• waste waters
• petrochemical residues
• toxicological residues

To check out SiliCycle's SiliaPrep SPE product line and the various sorbents available, visit: https://www.silicycle.com/products/si...

To see different applications and their protocols, you can find case studies in our Sample Prep Brochure: https://www.silicycle.com/media/broch...

posted by Babypillew6